The research group Host Septomics focuses on the host response during the course of severe infections and during the course of a sepsis. To analyze the host as well as the pathogen profile Host Septomics is using methods of functional genomics and proteomics and also the latest molecular- and cell biological techniques and defines systematically the transcriptome and protein pattern from healthy and infected cells and tissues.
A variety of sepsis relevant questions can be analyzed with the help of high-resolution mass spectrometry of LC-coupled ESI-MS-devices (Orbitrap Velos Pro and Orbitrap Fusion ETD):
• proteomic analysis of plasma samples
• analysis of the secretion from cellular proteins under inflammatory conditions
• analysis of subproteoms, e.g. glykoproteoms or phosphorproteoms
• analysis of protein-protein-interactions via immunoprecipitations or affinity chromatography
• label-free quantification of protein abundances
Two high efficient sequencers (Personal Genome Machine (PGM) and Ion Proton) enable the complex processing of different molecular biological questions:
• RNA-Sequencing (Transkriptome profiling)
The laboratories of Host Septomics (cell biological laboratory, microbiological laboratory and general functional laboratory) have a large-scale equipment of ultra-modern devices to analyse questions concerning the interaction between host and pathogen in the course of a sepsis.